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Enzyme is an   Eye opener for you!

  • Enzymes are proteins which are essential for life
  • It is a substance produced by living organism which acts as catalyst
  • In 1876, the term “enzyme” was coined by William Kuhne
  • In 1917, bacterial amylase obtained from Bacillus subtilis used for desizing in textile processing for the first time by Boidin & Effront
  • Enzyme activity = moles of substrate converted per unit time
  • Enzyme activity is denoted as “IU” – International Unit = amount of enzyme that will convert 1 mmol of substrate per minute in specified conditions

“SI” unit = katal = mol/sec (i.e) moles converted per second

  • Enzymes produced  by cell function within cells are called Endoenzymes
  • Enzymes liberated by living cells and catalyses reactions in cells environment are called Exoenzymes
  • An enzyme that, in the active state, contains one or more metal ions which are essential for its biological function
  • Minerals play one of their most important functions in the body when they participate in the metalloenzyme systems
  • The complete enzyme system is referred to as the holoenzyme
  • The protein portion of the holoenzyme is termed as “apoenzyme
  • Enzymes are protein molecules in cells which work as catalysts
  • Biological catalysts to accelerate chemical/biochemical reactions
  • It enable customer’s process eco-friendly, to produce more with less energy and to replace conventional chemical process

Factors affecting enzyme activity :

pH

Temperature

Product concentration

Enzyme Concentration

Activators or inhibitors

Substrate concentration

  • Optimum storage temperature is 35-40 degree Celsius
  • Optimum pH is 7
  • Enzymes are usually named after the substrate upon which they act by adding the suffix “-ase

Enzymes are being used in various fields closely related to real-life

  • Replacing conventional chemical industries and creating new markets with enzyme technology development
  • Single enzyme can be applied to various fields

Enzyme therapy          Bio-fuels               Detergents      Alternative Antibiotics      Food & Beverage    Pulp & Paper       Bio-pharmaceuticals      Starch & woody

                                        

        Bio-based chemicals     Wastewater treatment      Animal health & nutrition     Cosmetics & personal care       Leather                    Textile

                                           

                                                Gelatin hydrolysis        Lactose hydrolysis         Sugarcane industry          Nutraceuticals

                     

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